This study investigates the capacity of Deinbollia mosaic virus (DMV) to infect a restricted host range of Solanaceae and Euphorbiaceae species. Biolistic inoculation of Nicotiana benthamiana with concatemeric DNAs resulted in systemic infection associated with yellow mosaic symptoms, while DNA partial dimers caused asymptomatic systemic infection. DMV was not infectious to cassava (Manihot esculenta Crantz), suggesting host resistance to the virus. This study demonstrates the first experimental infectivity analysis of DMV in N. benthamiana and cassava.