Cassava brown streak disease (CBSD) is an economically important disease of cassava (Manihot esculenta Crantz) caused by Cassava brown streak virus (CBSV). Use of clean planting material is one of the strategies for disease management. However, obtaining clean planting material for some farmer-preferred varieties is often difficult. This research was aimed at evaluating the effect of meristem tip sizes, effects of varying concentration levels of ribavirin and salicylic acid and determining the efficacy of thermotherapy in combination with either meristem tip culture or chemotherapy in the elimination of CBSV from infected cassava. CBSV infected cuttings of Guzo variety collected from Coast province of Kenya were established and maintained in a greenhouse at the Plant Quarantine Station in the Kenya Plant Health Inspectorate Service in Muguga and were used as test plants. Cassava leaves were sampled from eighteen cassava plants of Guzo variety and virus indexing was done using Reverse Transcriptase-Polymerase Chain Reaction with virus specific primers. Those that tested positive for CBSV were used as initiation materials. From the in vitro plantlets established, the second sub-cultures were subjected to the virus elimination procedures. In vitro meristems (0.5mm, 1mm, 2mm and 10mm) were obtained and cultured in modified Murashige and Skoog media. For chemotherapy, nodes were cultured in MS media supplemented with antivirals at 0mg/l, 10mg/l, 20mg/l, 30mg/l. In the combination treatments single nodal plantlets were subjected to thermotherapy at 38°C for twenty one days, then excised meristem tips (1.0mm), with some plants being subjected to ribavirn treatments at (10mg/l, 20mg/l and 30mg/l).